Formation of Boundaries of Transcriptionally Silent Chromatin

  1. Based on the introduction of “Formation of Boundaries of Transcriptionally Silent Chromatin by Nucleosome-Excluding Structions,” give 3 models that might explain how boundary or insulator elements protect a gene from the influence of nearby genomic regions.

Link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC350542/

  1. Briefly describe the 3 assays the authors used in the aforementioned paper to demonstrate that LexA did not have gene activating activity.
  2. Briefly explain how each of the following is an example of position-effect variegation:
    1. Burkitt’s lymphoma,
  3. Alpha-thalassemia, and
  5. Describe how HpaII and MspI can be used to determine if a given DNA sequence is methylated. Be sure to mention positive and negative controls and any limitations associated with these enzymes.
  6. How could a Southern blot be used to determine if a given DNA sequence contains methylated CpGs?
  7. How does Figure 6 in “Amyloid-β Alters the DNA Methylation Status of Cell-Fate Genes in an Alzheimer’s Disease Model” support the model proposed in Figure 7
  8. Using Proverbs 22:6 and Ephesians 6:1–4, describe how these passages might apply to your current understanding of epigenetics and responsible parenting as outlined in the “low-licking rat” experiment

Link to bible reading: https://www.biblegateway.com/passage/?search=Ephesians+6%3A1-4%2CProverbs+22%3A6&version=NIV

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